Intermediate

Part:BBa_K300983

Designed by: Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-09)


BBa_K300000 construction intermediate (provided by Mr Gene)

This part has been provided by Mr Gene synthesis service and it has been used to construct BBa_K300982 plasmid. On the other hand, BBa_K300982 was used to construct BBa_K300000 BioBrick integrative base vector.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 635
    Illegal XbaI site found at 24
    Illegal XbaI site found at 805
    Illegal SpeI site found at 212
    Illegal PstI site found at 652
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 635
    Illegal NheI site found at 43
    Illegal NheI site found at 459
    Illegal SpeI site found at 212
    Illegal PstI site found at 652
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 635
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 635
    Illegal XbaI site found at 24
    Illegal XbaI site found at 805
    Illegal SpeI site found at 212
    Illegal PstI site found at 652
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 635
    Illegal XbaI site found at 24
    Illegal XbaI site found at 805
    Illegal SpeI site found at 212
    Illegal PstI site found at 652
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None